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IUL Biotechnology Series, 8 |
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by Terese M. Bergfors (Editor) |
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Edition: Second |
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Book
Details:
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| Editor's Review, March 15, 2009 |
| Reviewer: Terese Bergfors |
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Obtaining
good
quality protein crystals still remains a bottleneck in structural
determination by X-ray crystallography. This book is written for
biochemists, crystallographers, graduate students, technicians,
undergraduates or anyone else working with proteins with the intention
of crystallizing them. The approach is a practical one and no previous
experience of the laboratory techniques is assumed. It is suitable for
beginners in the field, but the extent of the material provides a
thorough coverage of the topic for experienced structural biologists.
If you already have the first edition of Protein
Crystallization, why should you buy this second edition? The first
edition was published in 1999. In the ten years since then, the field
of protein crystallization has experienced rapid development and
change. The second edition of the book contains ten new chapters
and the other seven have been revised and updated. The chapters have
been written by twenty-three scientists who are actively involved in
the development of methodology for protein crystallization. Laboratory
exercises are included which can be used for teaching purposes in
protein crystallization or crystallography courses. The exercises have
been tested in the "Practical Protein Crystallization" courses that
have been running since 1994 at Uppsala University (Sweden), as well as
courses elsewhere.
The book is divided into four separate sections: Methods,
Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There
are 30 color plates for identifying phenomena found in crystallization
drops. As in the first edition, the emphasis of the book is on
practical problems and questions encountered at the lab bench.
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Completely revised and updated, Protein
Crystallization, 2nd Edition is a greatly expanded follow-up to
the
best- selling 1st edition. Completely new chapters on high-through- put
methods, mass spectrometry, microcalorimetry, counter- diffusion,
heavy-atom derivatization, selenomethionine- labeling, rational
strategies for crystallization, and protein modification to improve
crystallization. Updated chapters on formulation of the protein before
crystallization, char- acterization of the protein by dynamic light
scattering, classic methods and the phase diagram, seeding, and
cryoprotection of the crystals. Thirty full-color plates for evaluating
crys- tallization drops. Separate section of laboratory exercises,
ideal
for crystallization courses. A–Z glossary.
Written by the best experts in protein crystallization from different countries.
Edited by Terese M. Bergfors, Dept. of Molecular Biology, Uppsala University, Sweden.
Reviews