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  A-Z of Quantitative RCR

IUL Biotechnology Series, 5

by Stephen A. Bustin (Editor) 

Edition: First



Print: Third

 

 


Book Details:
  • Series: IUL Biotechnology Series

  • Volume: 5

  • Binding: Hardcover 

  • Pages: 912

  • Dimensions (in inches): 1.75 x 9.50 x 6.50

  • Publisher: International University Line 

  • Publication Date: August, 2004

  • ISBN: 0-9636817-8-8

  • List Price: $119.95

  • Used Price: $109.95


Editorial Reviews
From Book News, Inc.
In review Book News, Inc., Portland, OR


 

 

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Look inside this book

Abstract:

This book is a comprehensive manual to allow both the novice researcher and the expert to set up and carry out quantitative PCR assays from scratch. However, this book also sets out to explain as many features of qPCR as possible, provide alternative viewpoints, methods, and aims to simulate the researchers into generating, interpreting, and publishing data that are reproducible, reliable, and biologically meaningful

     Written by the best experts in drug design from different countries.

      Edited by Stephen A. Bustin, Barts and The London Queen Mary's School of Medicine and Dentistry, London, United Kingdom.

 

 

Reviews


  Gregory L. Shipley, Ph.D., Director, Quantitative Genomics Core Laboratory, Department of Integrative Biology and Pharmacology. The University of Texas Health Science Center, Houston

 

The book, A-Z of Quantitative PCR edited by Dr. Stephen Bustin is, for once, exactly what the title claims, Everything you want and need to know about real-time quantitative PCR. Since its' inception in 1996, real-time quantitative PCR has grown dramatically in the assortment of available hardware, chemistries and uses for this extremely robust technology. However there has been no single reference to point new users to that completely covered all facets of this technology. The A-Z of Quantitative PCR fills that void. The material is presented in a very readable form for those new to the technique and yet has plenty of detailed information for experienced veterans. The book is devided into three sections beginning with three excellent overviews of the technique followed by several chapters covering the basic principles behind every aspect of preparing samples to performing the RT and PCRs to how the various assay chemistries and available instrumentation work. Finally, there are quite a few chapters devoted to specific applications of the technology. A real plus are the appendices covering many handy biochemical facts and glossary of terms for the newly initiated. All in all a very readable and informative book that should be a must read for enyone wanting to get into the field of real-time quantitative PCR.


 

David G. Ginzinger, Ph.D., Laboratory Director, Genome Analysis Core Facility, UCSF Comprehensive Cancer Center


 

Dr. Stephen Bustin has done a remarkable job on thr aptly titled A-Z of Quantitative PCR. It has remarkable depth to satisfy the needs of the most experienced Q-PCR scientist as well as the breadth to inspire the novice. Very clearly written and easy to use protocols and helpful tips ensure success when attempting your first real-time Q-PCR experiment. An amazingly rich resource for everything you wanted to know about real-time quantitative PCR--from the historical background to chemistried, instruments, and data analysis. This book will quickly become an essential reference manual for every lab wanting to perform quantitative PCR. If after reading this book and following recomendations you are not able to get Q-PCR to work, you should seriously consider changing careers.